The transgene insert contains the following elements: the 5' PiggyBAC transposon inverted repeat, 1027 bp sequence containing Atoh1 enhancer Eh2 or E3, Hsp68 minimal promoter, EGFP reporter gene, SV40 early poly(A) signal sequences, and the 3' transposon inverted repeat. The transgene was injected into single-cell zygotes together with PiggyBAC transposase mRNA to facilitate integration into the genome. The reporter is expressed in cochlear hair cells in newborn mice. (J:333396)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
CD-1
Transposon induced
Transposon insertion
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

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PMID
Journal
Year
IF
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