A loxP site was inserted upstream of exon 2 and a an FRT-flanked pgk-neo cassette and loxP site were inserted downstream of exon 2 via homologous recombination. Flp-mediated recombination removed the neo cassette. (J:217636)
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A loxP site was inserted upstream of exon 2 and a an FRT-flanked pgk-neo cassette and loxP site were inserted downstream of exon 2 via homologous recombination. Flp-mediated recombination removed the neo cassette. (J:217636)