Gt(ROSA)26Sor^{tm11(CAG-ZsGreen,-tdTomato)Bzsh}

A CAG-rox-stop-rox-ZsGreen-WPRE-pA-Frt-Neo-Frt sequence into exon1 of the Rosa26 locus and the insulator-CAG-loxP-stop-loxP-tdTomato-WPRE-pA sequence into exon2 of the Rosa26 gene locus using traditional targeted mutation through homologous recombination in embryonic stem cells. It is a Dre/rox and Cre/loxP dual reporter. With Dre recombinase activity, the first STOP sequence is excised and ZsGreen can be expressed. With Cre recombinase activity, the second STOP sequence is removed allowing the expression of tdTomato downstream. (J:285035)