The targeting construct inserted a loxP site upstream of exon 1 and an FRT-flanked neomycin resistance cassette with a 3' loxP site downstream of exon 1. Flp-mediated recombination removed the selection cassette and left exon 1 floxed. (J:333962)
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The targeting construct inserted a loxP site upstream of exon 1 and an FRT-flanked neomycin resistance cassette with a 3' loxP site downstream of exon 1. Flp-mediated recombination removed the selection cassette and left exon 1 floxed. (J:333962)