This transgene was created by coinjection of two overlapping genomic fragments: an NcoI-NcoI restriction fragment containing sequence 5' to and 3' from the Atoh1 coding sequence and a BglII-BglII fragment containing the last ~400 bp of coding sequence and 6 kb 3' of the coding region. Coding sequence from the N terminus of Drosophila Bride of Sevenless (BOSS) (TSPTKKSAPLRITKPQPTS) was inserted in-frame with the Atoh1 coding sequence after codon 8 to enable distinguishing endogenous from transgenic gene products. (J:60854)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
(C57BL/6 x DBA)F1
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Insertion
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--
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

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PMID
Journal
Year
IF
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