Atoh1 enhancer Eh2 or E3 was targeted with sgRNAs (targeting TATGAACTAGAATATTGTATTGG and AACGTATTCAAAGTCAAAGCGGG) using CRISPR/Cas9 technology, resulting in a 2519 bp deletion (chr6:64774536-64777054 (GRCm39)) including the enhancer. (J:332270, J:333396)