CRISPR-targeting inserted into the locus a CAG promoter driving expression of a FLAG-tag Streptococcus pyogenes dead Cas9 (dCas9) and FRT-flanked IRES and EGFP reporter gene. A floxed stop cassette prevents expression of the cas9 gene. (J:258204)
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CRISPR-targeting inserted into the locus a CAG promoter driving expression of a FLAG-tag Streptococcus pyogenes dead Cas9 (dCas9) and FRT-flanked IRES and EGFP reporter gene. A floxed stop cassette prevents expression of the cas9 gene. (J:258204)