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A 217-bp fragment containing the C-terminal tag sequences (fused in frame to the last Bhlhe41 codon) and a 1.9-kb fragment containing the mouse phosphoglycerate kinase (Pgk1) promoter linked to the neomycin-resistance gene (flanked by loxP sites) was inserted into the locus by homologous recombination in ES cells. The tag sequences contained epitopes for antibodies to Flag and V5, two cleavage sites for the TEV protease, and a biotin acceptor sequence (Biotin) for biotinylation by the E. coli biotin ligase BirA. Lox-flanked neo-cassette was removed by cre recombinase. (J:258170)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
