Tg(Crhr2-EGFP/cre)#And

A mouse bacterial artificial chromosome (BAC clone RP23-78P13) containing the entire Crfr2 gene was used to generate the final transgene. With the BAC another gene Inmt was disrupted by inserting a bovine growth hormone polyadenylation sequence (BGHpA) cassette into the 5' end of the Inmt gene that removed the start codon. To simultaneously target Crfr2a and suppress expression Crfr2b from the BAC, a cassette containing an eGFPCre fusion gene followed by an SV40pA was inserted into the 5'UTR of the third exon of the Crfr2 gene, and a STOP codon was introduced into exon IV (shared by both a and b splice forms) to terminate translation of any Crfr2b mRNA transcribed from the BAC. Founder information and line numbers were not available. The pound symbol (#) is used when no line is specified and/or lines are pooled. (J:333398)