The targeting vector is designed to replace the endogenous STOP codon of the gene with a TimeSTAMP cassette with a STOP codon, and an FRT-flanked neomycin (neo) cassette. The TimeSTAMP (Time-Specific Tagging for the Age Measurement of Proteins) cassette contains, from 5' to 3';, N-terminal Venus fragment, a cis-cleaving hepatitis C virus (HCV) NS3 protease, C-terminal Venus fragment, and two hemagglutinin (2xHA) tags. Flp-mediated recombination removed the neomycin cassette. (J:101977)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
B6.Cg-Thy1a
Targeted
Insertion
--
1
--
--

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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