The single exon was replaced with a loxP-flanked Junb exon and an FRT-flanked pgk-neomycin cassette. The neomycin resistance cassette was removed via flp-mediated recombination, leaving the entire open reading frame floxed. (J:287427)
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The single exon was replaced with a loxP-flanked Junb exon and an FRT-flanked pgk-neomycin cassette. The neomycin resistance cassette was removed via flp-mediated recombination, leaving the entire open reading frame floxed. (J:287427)