A loxP site and FRT-flanked neomycin resistance gene cassette was inserted upstream of exon 2 and a second loxP site was inserted downstream of exon 3. The neomycin gene was removed via flp-mediated recombination leaving exons 2-3 floxed. (J:287473)
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A loxP site and FRT-flanked neomycin resistance gene cassette was inserted upstream of exon 2 and a second loxP site was inserted downstream of exon 3. The neomycin gene was removed via flp-mediated recombination leaving exons 2-3 floxed. (J:287473)