In a step wise targeting process a floxed neo cassette was inserted downstream of exon 8. This was followed by removal of the neo leaving a single loxP site. Then an FRT flanked neo cassette and loxP site were inserted upstream of exon 5. Flp mediated recombination removed the neo leaving exons 5 -8 floxed. (J:321165)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count