A construct containing an FRT site, splice acceptor, IRES, beta-gal gene, loxP site, PGK-neo cassette, FRT site, and loxP site was inserted upstream of exon 2 and a third loxP site was inserted downstream of exon 2 via homologous recombination. Flp mediated recombination removed the bet-gal and neo cassettes leaving exon 2 floxed. (J:319656)
Basic Information
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count