A loxP site was inserted upstream of exon 5 and an FRT-flanked neomycin selection cassette and second loxP site were inserted downstream of the 3UTR. Exons 5 to 7 and the 3UTR were deleted via cre-mediated recombination. (J:327648)
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A loxP site was inserted upstream of exon 5 and an FRT-flanked neomycin selection cassette and second loxP site were inserted downstream of the 3UTR. Exons 5 to 7 and the 3UTR were deleted via cre-mediated recombination. (J:327648)