An FRT-flanked PGK-neomcyin selection cassette and loxP site were inserted upstream of exon 4. A second loxP site was inserted downstream of exon 5. The neomycin selection gene was removed via flp-mediated recombination leaving exons 4-5 floxed. (J:301391)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count