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A construct containing a CAG promoter, floxed stop cassette, tdTomato CDS, 3X Mir29-3p tough decoy, and WPRE was inserted in the gene via CRISPR-cas9 mediated recombination. The Mir29-3p tough decoy contains binding sites for the members of the Mir29 family including Mir29a, Mir29b-1, Mir29b-2, and Mir29c. Cre mediated recombination removed the floxed stop cassette.qRT-PCR analysis found a 30-60% decrease in levels in the calvaria of mice carrying the allele. (J:313732)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
