A loxP site was inserted upstream of exon 4 and a loxP site and FRT-flanked pgk-neo cassette were inserted downstream of exon 5 via homologous recombination. Flp-mediated recombination removed the pgk-neo cassette. (J:322066)
查看原文 参与反馈
A loxP site was inserted upstream of exon 4 and a loxP site and FRT-flanked pgk-neo cassette were inserted downstream of exon 5 via homologous recombination. Flp-mediated recombination removed the pgk-neo cassette. (J:322066)