A loxP site, FRT-flanked PGK-neomycin cassette, and second loxP site were inserted upstream of exon 2 and a third loxP site was inserted downstream of exon 2. The neomycin cassette was removed via flp-mediated recombination leaving exon 2 floxed. (J:271769)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count