Cnr2^tm1.2Geno

An enhanced green fluorescent protein reporter gene (EGFP) preceded by an internal ribosomal entry site (IRES) and followed by an FRT-flanked neomycin gene was inserted into the 3 untranslated region (UTR) of Cnr2. This results in the expression of the EGFP reporter under the control of the endogenous mouse Cnr2 promoter and transcript from the same bicistronic mRNA as the CB2R protein. In addition, exon 3 and the reporter (including the neomycin) are flanked by loxP sites, allowing for conditional inactivation of the gene in cells expressing cre recombinase. The neomycin gene was removed via flp-mediated recombination. Exon 3 and the EGFP were deleted via cre-mediated recombination. (J:273502)