An Ametrine-2A and FlpO (a codon-optimized flippase) cDNS followed by a 2A peptide linker was inserted after the ATG start codon of the Foxp3 gene with a AttB-Neo-AttP cassette. Germline mice were then crossed with Rosa.PhiC31 mice to remove the Neo cassette. (J:312516)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count