A loxP site was inserted upstream of exon 4 and an FRT-flanked neomycin selection cassette and second loxP site were inserted downstream of exon 4. The neomycin cassette was removed via flp-mediated recombination leaving exon 4 floxed. (J:329181)
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A loxP site was inserted upstream of exon 4 and an FRT-flanked neomycin selection cassette and second loxP site were inserted downstream of exon 4. The neomycin cassette was removed via flp-mediated recombination leaving exon 4 floxed. (J:329181)