A 6.3 kb sequence, containing the border (insulator) region between the Kcnj and Sox9 topologically associated domains (TADs), was targeted for insertion in Rr325em1Smun Rr325 deletion allele ES cells about 125 kb upstream of Sox9 with an sgRNA (targeting GGAGGGACACTGATATTTCT) using CRISPR/Cas9 technology. (J:282642)
Basic Information
(129S6/SvEvTac x C57BL/6NCrl)F1
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count