A loxP site was inserted 5' of the coding region and a loxP site and FRT-flanked neo cassette were inserted 3' of the coding region via homologous recombination. Flp-mediated recombination removed the neo cassette. (J:330572)
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A loxP site was inserted 5' of the coding region and a loxP site and FRT-flanked neo cassette were inserted 3' of the coding region via homologous recombination. Flp-mediated recombination removed the neo cassette. (J:330572)