CRISPR/cas9 endonuclease-mediated homology-directed repair (HDR) genome editing including two guide RNAs (CACCgTACACGCTATATTTGTGCTA; AAACTAGCACAAATATAGCGTGTAc and CACCgTACAGACTGTAGTTTAGCTT; AAACAAGCTAAACTACAGTCTGTA) is used to insert loxP sites flanking the CpG island (CGI) located at the 5prime portion of the region. (J:331751)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count