CRISPR/cas9 endonuclease-mediated homology-directed repair (HDR) genome editing including two guide RNAs (CACCgTACACGCTATATTTGTGCTA; AAACTAGCACAAATATAGCGTGTAc and CACCgTACAGACTGTAGTTTAGCTT; AAACAAGCTAAACTACAGTCTGTA) is used to insert loxP sites flanking the CpG island (CGI) located at the 5prime portion of the region. (J:331751)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
(C57BL/6 x DBA/2)F1
Endonuclease-mediated
Insertion
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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