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Exons 2 and 3 were flanked by loxP sites and a neomycin-resistance cassette flanked by FRT sites was inserted between exons 1 and 2. To prevent missplicing, a putative splice acceptor site downstream of the neomycin cassette was mutated (gt to ca) and an additional EcoRV-site was introduced adjacent to the second loxP site to aid in Southern blot detection. The neomycin cassette was removed via flp-mediated recombination. (J:331730)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
