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BAC clone CH29-40010 containing the mouse Mir22 gene was manipulated through Red/ET recombination technology. A loxP was inserted in the 5 flanking region of exon 1 and a second loxP site was introduced in the 3 flanking region of exon 1 with a PGK-FRT-Neo-FRT-loxP cassette. Targeted ES cells were re-electroporated with a flp-recombinase expressing plasmid to remove the neomycin cassette leaving exon 1 floxed. Exon 1 was deleted via cre-mediated recombination in the germline. RT-qPCR confirmed undetectable levels of mature miR-22-3p in the liver, epididymal white adipose tissue and gastrocnemius tissue. (J:331713)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
