Ythdc1^{tm1c(EUCOMM)Wtsi}

The L1L2_gt1 cassette was inserted at position 86967482 of Chromosome 5 upstream of the critical exons 5-7 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exons at position 86969487. The critical exons 5-7 are thus flanked by loxP sites. Flp-mediated recombination removed the lacZ/neomycine leaving exons 5-7 floxed. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:331492)