A HpaI-NcoI restriction fragment containing the core Ebeta Tcrb enhancer was replaced with a loxP site flanked neomycin resistance gene cassette. The neo cassette was removed through subsequent cre-mediated recombination. (J:34312)
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A HpaI-NcoI restriction fragment containing the core Ebeta Tcrb enhancer was replaced with a loxP site flanked neomycin resistance gene cassette. The neo cassette was removed through subsequent cre-mediated recombination. (J:34312)