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The targeting construct introduced the tdiRFP713NLS reporter gene expressed under CAG promoter and WPRE sequence into 3 UTR region. Both cre- and flp-mediated recombination is required to achieve expression of the reporter. FRT-Stop-FRT cassette was removed by crossing pCAG-FLPe strain. Then, loxP-Stop-loxP cassette was removed by injecting pIC-Cre expression plasmid to the fertilized eggs. Therefore, tdiFP713NLS is expressed by all cells throughout the body. The HA-epitope tga was is attched to tdiFP713NLS. (J:104881)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
