A loxP site was inserted upstream of exon 1 and a loxP and FRT flanked neo cassette was inserted downstream of exon 2 via homologous recombination. Flp-mediated recombination removed the neo cassette leaving exons 1 and 2 floxed. (J:242006)
查看原文 参与反馈
A loxP site was inserted upstream of exon 1 and a loxP and FRT flanked neo cassette was inserted downstream of exon 2 via homologous recombination. Flp-mediated recombination removed the neo cassette leaving exons 1 and 2 floxed. (J:242006)