CRISPR-targeting inserted an mCherry reporter gene downstream of the coding sequence. A T to A silent mutation was introduced into the position of Rtl5 Threonine 593 (ACT to ACA) to inhibit recutting of the genomic DNA after editing (J:329352)
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CRISPR-targeting inserted an mCherry reporter gene downstream of the coding sequence. A T to A silent mutation was introduced into the position of Rtl5 Threonine 593 (ACT to ACA) to inhibit recutting of the genomic DNA after editing (J:329352)