A loxP site was inserted upstream of the Zic3 mesodermal enhancer and a neomycin resistance gene cassette and a second loxP site downstream. The enhancer and neo cassette were deleted through subsequent cre-mediated recombination. (J:310742)
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A loxP site was inserted upstream of the Zic3 mesodermal enhancer and a neomycin resistance gene cassette and a second loxP site downstream. The enhancer and neo cassette were deleted through subsequent cre-mediated recombination. (J:310742)