CRISPR/cas9 genome editing is used to insert a 5' HA tag, a membrane anchored eGFP repair plasmid (eGFPcaax), a STOP sequence, a bGH polyA sequence and a 3' HA tag into the 3' end of the exon2 locus, and a non-targeting PGK-puromycin plasmid. The endogenous promoter drives eGFP expression. (J:332176)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6NTac
Endonuclease-mediated
Insertion
--
1
6
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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