CRISPR/cas9 genome editing is used to insert a 5' HA tag, a membrane anchored eGFP repair plasmid (eGFPcaax), a STOP sequence, a bGH polyA sequence and a 3' HA tag into the 3' end of the exon2 locus, and a non-targeting PGK-puromycin plasmid. The endogenous promoter drives eGFP expression. (J:332176)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count