CRISPR/cas9 genome editing uses guide RNAs, targeting DNA sequence 5';-aagctaggactttaatactc-3', to insert a loxP flanked STOP cassette into intron 4. Plk4-201 was used as reference for the exon number and the guide sequences. (J:329155)
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CRISPR/cas9 genome editing uses guide RNAs, targeting DNA sequence 5';-aagctaggactttaatactc-3', to insert a loxP flanked STOP cassette into intron 4. Plk4-201 was used as reference for the exon number and the guide sequences. (J:329155)