A loxp flanked TK/neomycin selection cassette was inserted upstream of exon 2 and a third loxP site was inserted downstream of the exon 2 coding region within the non-coding region of the exon. The selection cassette was removed via cre-recombinase transfection in ES cells leaving exon 2 floxed. (J:269358)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count