Rr270^em1Mgn

The conserved region, upstream of transcription start site 1 (TSS1) in the 5' UTR of Sox17 exon 1, was targeted with crRNAs (targeting TTTCTTCACTAAATGGGTGTTGG and ACTAGAGATTTACTGGGGTGGGG), tracrRNA and an ssODN template (GATACTTTGGGAGGAGTGTTGGGGCCAACCGCACTTCAGACCCGCCAGCAGTGTGAGTGGGCCATATTTCAGTTTCTTCACTAAATGGTGGGGTGGGGGTGGGTATCTGGACTAGAAGCCTGGCACGCCTGTCTGCTGTAGTCCGCAGCGGTATCACACTCAAACAGTGCAGGATTTCTA) using CRISPR/Cas9 technology, leading to a 117 bp deletion. (J:328045)