LoxP sites were inserted into intron 1 and 3' of WT exon 2. An FRT site flanked selection cassette that was inserted was removed via subsequent Flp-mediated recombination. Cre-mediated recombination deleted the floxed region. (J:328121)
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LoxP sites were inserted into intron 1 and 3' of WT exon 2. An FRT site flanked selection cassette that was inserted was removed via subsequent Flp-mediated recombination. Cre-mediated recombination deleted the floxed region. (J:328121)