A loxP site was inserted upstream of exon 8 and an frt-flanked PGK-neo cassette and second loxP site were inserted downstream of exon 8. The neomycin selection cassette was removed via flp-mediated recombination. (J:328115)
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A loxP site was inserted upstream of exon 8 and an frt-flanked PGK-neo cassette and second loxP site were inserted downstream of exon 8. The neomycin selection cassette was removed via flp-mediated recombination. (J:328115)