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This allele was generated by a 3' loxP site insertion in intron 5 via electroporation of C57BL/6J zygotes of CRISPR/Cas9 RNP (sgRNA sequence TTGTGACCATTTGTCTGCAA; chr11:118,287,207-118,287,226 GRCm39/mm39) and a loxP-containing ssDNA oligonucleotide (200bp). Subsequent CRISPR/Cas9-targeting with RNPs (sgRNA sequence CAATGTCCCGTTGCTAAGTG - Chr11:118289852-118289871 GRCm39/mm39) and a ssDNA oligonucleotide (200bp) inserted the 5' loxP site in intron 2, to generate the final conditional mouse model. (J:329329)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
