Foxm1^em1Pra

Using CRISPR/Cas9 technology, the point mutations were introduced to change the transcription activator region of the Foxm1 gene corresponding residues in the mouse genome to aspartic acid. A phosphomimetic mutant, FoxM1DD, in which the two Plk1 phosphorylation sites were replaced by aspartic acid, retains the transcriptional activator function but fails to repress the mammary differentiation genes Gata3 and FoxA1. The founder 10 containing the mutations, FoxM1S727 mutated to aspartic acid where S727 corresponds to human S715, and FoxM1S736 mutated to aspartic acid where S736 corresponds to human S724, were used for the study. (J:326291)