Mymx^em#Eno

CRISPR/Cas9 genome editing technology was used to generate deletions in the gene. The cutting sites of the two sgRNAs were 122 base pairs apart in the third exon. PCR with primers amplifying this region showed mutant mice with deletions that ranged from 163 to 470 bp, reflecting different indels. The lines were not distinguished in the reporting of data in the original reference, and thus the nomenclature includes a # sign to indicate pooled data. (J:242061)