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A targeting vector is designed to insert FRT-flanked PGK-Neo-promoterless, ATG-less hygromycin cassette 500 bp downstream of the 3' UTR of the Col1a1 locus. Flpe recombinase is then used to FLP-IN a tetO-Plk4-EYFP plasmid. The Plk4-EYFP plasmid contained mouse Plk4 open reading frame (ORF) C-terminally tagged with EYFP downstream of the tetracycline response element (tetO) and CMV minimal promoter. (J:272389)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
