CRISPR/Cas9 technology using gRNAs 5'-ggatgcagctaaggctttgg-3' and 5'-gacttctggtgacatatctt-3' created a large fragment knockout. Western blot analysis confirmed the absence of protein expression in the testes of homozygous mutant males. (J:322392)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count