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CRISPR/Cas9 endonuclease-mediated homology-directed repair (HDR) genome editing is used to insert an mCherry reporter in-frame before the termination codon. A combination of two sgRNAs (sgRNA1 ATTCTTATAGAGGACGCACT and sgRNA2 AAGTGCGTCCTCTATAAGAA or sgRNA1 and sgRNA3 AACATTGTGGTGGCTGATAT) is used to traget the junction between exon 4 and the 3' untranslated region (UTR). (J:302430)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
