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Two guide RNAs (5'-TCATCATCGCGTTGACGGAT-3', PAM=GGG; 5'- GACGGATGGGGAGCTGCACG-3', PAM=AGG) and a single-stranded DNA containing the E150V mutation is used to create a GAG to GTA missense mutation. A silent mutation was also added, creating a ScaI restriction site (AGTACT). This mutation hinders the binding of ANTRX1 to collagen. (J:331389)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
