Gt(ROSA)26Sor^{tm1(CAG-EGFP,-BphP1/mCherry/TetR)Vvv}

The targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a loxP site, an enhanced green fluorescent protein sequence followed by an SV40 polyA signal, a loxP site, the RpBphP1/mCherry/TetR fusion protein (containing the sequences encoding a purple photosynthetic bacterium Rhodopseudomonas palustris-derived bacteriophytochrome photoreceptor P1 [RpBphP1], the monomeric red fluorescent protein [mCherry] and the tet repressor sequence [TetR]), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a bovine growth hormone polyA sequence (BGHpA) and an FRT-flanked neomycin resistance cassette. This entire targeting vector was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus via electroporation. (J:325201)