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Site-directed mutagenesis and PCR were used to add His and FLAG epitopes (ASGHHHHHHGMDYKDDDDKGLG) to the c-teminus of a cDNA for rat GABAA receptor 2 subunit (GABAAR2), short form. Enhanced YFP was inserted between amino acids 4 and 5 of the mature 2 peptide coding sequence. A mouse Thy1 promoter drives expression of the construct. (J:204699)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
