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ligonucleotides containing the Pax3 consensus binding site were multimerized. The sequence of the oligonucleotides is as follows: CSSPax3-A, 5-CCTCGTCACGCTTCGAATGT-3; and CSSPax3-B, 5-GGGACATTCGAAGCGTGACGA-3. The oligonucleotides were annealed and polymerized. Concatenamers containing five binding sites were gel-purified, and cloned into a TK-nLacZpA vector, which contains the nlacZ reporter gene under the control of the thymidine kinase (TK) promoter, into an SfiI site located 5 to the TK minimal promoter. A 7.4-kb plasmid fragment for injection was isolated using XhoI and NotI. (J:82809)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
