Mlh3^em2Jcs

Using an sgRNA (targeting GCTCCAAACGAATGCGTTCA) and an ssODN template (CTTAAATCTCCTCTAACTACAGACAGATACTTACCAGTAATAAGCTGCTCCAAACGTATGTGTTCATGTGCAGCATGCTGGTCCACCAGGACTAACAGGTTTCCACCTACAAAATAATCC) with CRISPR/Cas9 technology, arginine codon 1192 (CGC) was changed to histidine (CAC) (c.3575G>A, p.R1192H). This is the equivalent of the human p.R1230H mutation. (J:324056)